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luthy control vectors  (Addgene inc)


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    Addgene inc luthy control vectors
    Characterization of the TBK1 E696K variant. (A) Scheme of <t>LuTHy-BRET</t> assay to investigate binding of wt TBK1 and TBK1 E696K to optineurin (OPTN) or the TRAF family member–associated NF-κB activator (TANK) in live HEK293 cells. (B and C) Binding of wt TBK1- and TBK1 E696K -mCitrine-Protein <t>A</t> <t>(-mCit-PA)</t> to NanoLuc (NL)-tagged optineurin (B) or TANK (C) in LuTHy-BRET donor saturation assays. (D) Quantification of cBRET signals from TBK1 binding assays. PA-mCit-NL tandem construct shown as positive and cotransfection of single NL- and PA-mCit-tags as negative BRET controls. Relative BRET ratios were obtained by normalizing the BRET signal of optineurin or TANK with each TBK1 mutant to the interaction signals with wt TBK1, respectively. Mean ± SEM of n = 4 technical replicates per condition from three independent experiments; one-way ANOVA with Sidak’s post hoc test; **P < 0.01. (E) Comparison of wt TBK1- and TBK1 E696K -mCit-PA fusion protein expression as measured from fluorescence intensities. Mean ± SEM of n = 7 technical replicates per condition from three independent experiments; Student’s t test. (F) Western blot showing expression of the TBK1 mutants in HEK293 cells after transfection. Source data are available for this figure: .
    Luthy Control Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luthy control vectors/product/Addgene inc
    Average 93 stars, based on 10 article reviews
    luthy control vectors - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice"

    Article Title: A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20221190

    Characterization of the TBK1 E696K variant. (A) Scheme of LuTHy-BRET assay to investigate binding of wt TBK1 and TBK1 E696K to optineurin (OPTN) or the TRAF family member–associated NF-κB activator (TANK) in live HEK293 cells. (B and C) Binding of wt TBK1- and TBK1 E696K -mCitrine-Protein A (-mCit-PA) to NanoLuc (NL)-tagged optineurin (B) or TANK (C) in LuTHy-BRET donor saturation assays. (D) Quantification of cBRET signals from TBK1 binding assays. PA-mCit-NL tandem construct shown as positive and cotransfection of single NL- and PA-mCit-tags as negative BRET controls. Relative BRET ratios were obtained by normalizing the BRET signal of optineurin or TANK with each TBK1 mutant to the interaction signals with wt TBK1, respectively. Mean ± SEM of n = 4 technical replicates per condition from three independent experiments; one-way ANOVA with Sidak’s post hoc test; **P < 0.01. (E) Comparison of wt TBK1- and TBK1 E696K -mCit-PA fusion protein expression as measured from fluorescence intensities. Mean ± SEM of n = 7 technical replicates per condition from three independent experiments; Student’s t test. (F) Western blot showing expression of the TBK1 mutants in HEK293 cells after transfection. Source data are available for this figure: .
    Figure Legend Snippet: Characterization of the TBK1 E696K variant. (A) Scheme of LuTHy-BRET assay to investigate binding of wt TBK1 and TBK1 E696K to optineurin (OPTN) or the TRAF family member–associated NF-κB activator (TANK) in live HEK293 cells. (B and C) Binding of wt TBK1- and TBK1 E696K -mCitrine-Protein A (-mCit-PA) to NanoLuc (NL)-tagged optineurin (B) or TANK (C) in LuTHy-BRET donor saturation assays. (D) Quantification of cBRET signals from TBK1 binding assays. PA-mCit-NL tandem construct shown as positive and cotransfection of single NL- and PA-mCit-tags as negative BRET controls. Relative BRET ratios were obtained by normalizing the BRET signal of optineurin or TANK with each TBK1 mutant to the interaction signals with wt TBK1, respectively. Mean ± SEM of n = 4 technical replicates per condition from three independent experiments; one-way ANOVA with Sidak’s post hoc test; **P < 0.01. (E) Comparison of wt TBK1- and TBK1 E696K -mCit-PA fusion protein expression as measured from fluorescence intensities. Mean ± SEM of n = 7 technical replicates per condition from three independent experiments; Student’s t test. (F) Western blot showing expression of the TBK1 mutants in HEK293 cells after transfection. Source data are available for this figure: .

    Techniques Used: Variant Assay, Bioluminescence Resonance Energy Transfer, Binding Assay, Construct, Cotransfection, Mutagenesis, Comparison, Expressing, Fluorescence, Western Blot, Transfection



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    luthy control vectors  (Addgene inc)
    93
    Addgene inc luthy control vectors
    Characterization of the TBK1 E696K variant. (A) Scheme of <t>LuTHy-BRET</t> assay to investigate binding of wt TBK1 and TBK1 E696K to optineurin (OPTN) or the TRAF family member–associated NF-κB activator (TANK) in live HEK293 cells. (B and C) Binding of wt TBK1- and TBK1 E696K -mCitrine-Protein <t>A</t> <t>(-mCit-PA)</t> to NanoLuc (NL)-tagged optineurin (B) or TANK (C) in LuTHy-BRET donor saturation assays. (D) Quantification of cBRET signals from TBK1 binding assays. PA-mCit-NL tandem construct shown as positive and cotransfection of single NL- and PA-mCit-tags as negative BRET controls. Relative BRET ratios were obtained by normalizing the BRET signal of optineurin or TANK with each TBK1 mutant to the interaction signals with wt TBK1, respectively. Mean ± SEM of n = 4 technical replicates per condition from three independent experiments; one-way ANOVA with Sidak’s post hoc test; **P < 0.01. (E) Comparison of wt TBK1- and TBK1 E696K -mCit-PA fusion protein expression as measured from fluorescence intensities. Mean ± SEM of n = 7 technical replicates per condition from three independent experiments; Student’s t test. (F) Western blot showing expression of the TBK1 mutants in HEK293 cells after transfection. Source data are available for this figure: .
    Luthy Control Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luthy control vectors/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    luthy control vectors - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    pcdna3 1 luthy destination and control vectors  (Addgene inc)
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    Addgene inc pcdna3 1 luthy destination and control vectors
    Reagents and tools.
    Pcdna3 1 Luthy Destination And Control Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna3 1 luthy destination and control vectors/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    pcdna3 1 luthy destination and control vectors - by Bioz Stars, 2026-05
    92/100 stars
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    Characterization of the TBK1 E696K variant. (A) Scheme of LuTHy-BRET assay to investigate binding of wt TBK1 and TBK1 E696K to optineurin (OPTN) or the TRAF family member–associated NF-κB activator (TANK) in live HEK293 cells. (B and C) Binding of wt TBK1- and TBK1 E696K -mCitrine-Protein A (-mCit-PA) to NanoLuc (NL)-tagged optineurin (B) or TANK (C) in LuTHy-BRET donor saturation assays. (D) Quantification of cBRET signals from TBK1 binding assays. PA-mCit-NL tandem construct shown as positive and cotransfection of single NL- and PA-mCit-tags as negative BRET controls. Relative BRET ratios were obtained by normalizing the BRET signal of optineurin or TANK with each TBK1 mutant to the interaction signals with wt TBK1, respectively. Mean ± SEM of n = 4 technical replicates per condition from three independent experiments; one-way ANOVA with Sidak’s post hoc test; **P < 0.01. (E) Comparison of wt TBK1- and TBK1 E696K -mCit-PA fusion protein expression as measured from fluorescence intensities. Mean ± SEM of n = 7 technical replicates per condition from three independent experiments; Student’s t test. (F) Western blot showing expression of the TBK1 mutants in HEK293 cells after transfection. Source data are available for this figure: .

    Journal: The Journal of Experimental Medicine

    Article Title: A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice

    doi: 10.1084/jem.20221190

    Figure Lengend Snippet: Characterization of the TBK1 E696K variant. (A) Scheme of LuTHy-BRET assay to investigate binding of wt TBK1 and TBK1 E696K to optineurin (OPTN) or the TRAF family member–associated NF-κB activator (TANK) in live HEK293 cells. (B and C) Binding of wt TBK1- and TBK1 E696K -mCitrine-Protein A (-mCit-PA) to NanoLuc (NL)-tagged optineurin (B) or TANK (C) in LuTHy-BRET donor saturation assays. (D) Quantification of cBRET signals from TBK1 binding assays. PA-mCit-NL tandem construct shown as positive and cotransfection of single NL- and PA-mCit-tags as negative BRET controls. Relative BRET ratios were obtained by normalizing the BRET signal of optineurin or TANK with each TBK1 mutant to the interaction signals with wt TBK1, respectively. Mean ± SEM of n = 4 technical replicates per condition from three independent experiments; one-way ANOVA with Sidak’s post hoc test; **P < 0.01. (E) Comparison of wt TBK1- and TBK1 E696K -mCit-PA fusion protein expression as measured from fluorescence intensities. Mean ± SEM of n = 7 technical replicates per condition from three independent experiments; Student’s t test. (F) Western blot showing expression of the TBK1 mutants in HEK293 cells after transfection. Source data are available for this figure: .

    Article Snippet: LuTHy control vectors expressing only NL (#113442; Addgene) or PA-mCit ( #113443; Addgene) were used for the calculation of corrected scores, the PA-mCit-NL tandem construct (#113444; Addgene) as positive, and NL cotransfected with PA-mCit-only as negative controls.

    Techniques: Variant Assay, Bioluminescence Resonance Energy Transfer, Binding Assay, Construct, Cotransfection, Mutagenesis, Comparison, Expressing, Fluorescence, Western Blot, Transfection

    Reagents and tools.

    Journal: Molecular Systems Biology

    Article Title: AI-guided pipeline for protein–protein interaction drug discovery identifies a SARS-CoV-2 inhibitor

    doi: 10.1038/s44320-024-00019-8

    Figure Lengend Snippet: Reagents and tools.

    Article Snippet: pcDNA3.1 LuTHy destination and control vectors , Addgene (Trepte et al, ) , 113442–113449.

    Techniques: Recombinant, Control, Sequencing, Cell Culture, Binding Assay, Protease Inhibitor, Software